General Information

  • ID:  hor005184
  • Uniprot ID:  P81016
  • Protein name:  Vasoactive intestinal peptide
  • Gene name:  vip
  • Organism:  Pelophylax ridibundus (Marsh frog) (Rana ridibunda)
  • Family:  Glucagon family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Pelophylax (genus), Ranidae (family), Ranoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005184 neuropeptide hormone activity
  • GO BP:  GO:0007165 signal transduction; GO:0048255 mRNA stabilization; GO:0070459 prolactin secretion
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  HSDAVFTDNYSRFRKQMAVKKYLNSVLT
  • Length:  28
  • Propeptide:  HSDAVFTDNYSRFRKQMAVKKYLNSVLT
  • Signal peptide:  NA
  • Modification:  T28 Threonine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.
  • Mechanism:  Adenylate cyclase activation via G protein-coupled receptor
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: 1.2*10(-6）M
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P81016-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-P81016-F1.pdbhor005184_AF2.pdbhor005184_ESM.pdb

Physical Information

Mass: 380155 Formula: C148H232N42O43S
Absent amino acids: CEGIPW Common amino acids: KSV
pI: 10.32 Basic residues: 6
Polar residues: 9 Hydrophobic residues: 9
Hydrophobicity: -58.93 Boman Index: -6914
Half-Life: 3.5 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 66.07
Instability Index: 2803.57 Extinction Coefficient cystines: 2980
Absorbance 280nm: 110.37

Literature

  • PubMed ID:  7540547
  • Title:  Frog vasoactive intestinal polypeptide and galanin: primary structures and effects on pituitary adenylate cyclase.